ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of KIF18A gene protein in gastric cancer tissues and its association with the prognosis of patients.</p><p><b>METHODS</b>Twenty fresh paired gastric cancer specimens and adjacent normal mucosa(at least 5 cm from the edge of tumor) from 20 gastric cancer patients undergoing operation in Department of General Surgery at the First Affiliated Hosptial of Anhui Medical University between March 2015 and July 2015 were collected. Real-time PCR was used to examine KIF18A mRNA expression in above specimens. Meanwhile, paraffin embedded cancer tissue samples from 129 gastric cancer patients undergoing operation and 23 samples of randomly selected normal gastric tissue(adjacent non-cancer tissue) were collected to establish the microarray. Immunohistochemistry method was applied to detect the KIF18A protein expression in the microarray after confirmation by pathologists. Association of KIF18A expression with clinicopathological features in gastric cancer patients was evaluated. Cox proportional hazard model was used to identify prognostic risk factors.</p><p><b>RESULTS</b>Among 20 fresh paired gastric cancer specimens, mRNA expression of KIF18A in 16 specimens was obviously lower than that in adjacent normal tissues. The positive rate of KIF18A protein expression in gastric cancer tissues was significantly lower than that in normal gastric tissues in microarray[45.0%(58/129) vs. 69.6%(16/23), P=0.041]. KIF18A protein expression was significantly associated with invasion depth (P=0.008) and TNM staging (P=0.032). The median overall survival of all the 129 patients was 44.0(95% CI: 39.78-49.24) months. The three-year survival rates of patients with high and low KIF18A expression were 67.2% and 36.6% respectively(P=0.020). Cox regression analysis showed that KIF18A expression was an independent protective factor of the prognosis of gastric cancer patients (HR=0.570, 95% CI:0.335 to 0.970).</p><p><b>CONCLUSIONS</b>KIF18A expression is down-regulated in gastric cancer tissue, which may play a critical role in gastric cancer carcinogenesis. Lower expression of KIF18A is associated with poor prognosis of gastric cancer patients. KIF18A may be a potential prognostic marker of gastric cancer.</p>
Subject(s)
Humans , Biomarkers, Tumor , Metabolism , Immunohistochemistry , Kinesins , Metabolism , Neoplasm Staging , Prognosis , Proportional Hazards Models , Real-Time Polymerase Chain Reaction , Regression Analysis , Stomach Neoplasms , Diagnosis , Metabolism , Survival RateABSTRACT
OBJECTVE:To study antibacterial and anti-inflammatory effect of Maqin zhike syrup. METHODS:In vitro antibacterial test, mice ear swelling test and mice abdominal capillary permeability test were adopted to observe the antibacterial and anti-inflammatory effect of Maqin zhike syrup. RESULTS:Maqin zhike syrup could inhibit gram-negative and gram-positive bacteria, mice ear swelling, and reduce abdominal capillary permeability. It also enhanced humoral and cellular immunity. CONCLUSION:Maqin zhike syrup has the effect on bacterials, inflammatory and immunity with high safety.
ABSTRACT
OBJECTIVE: To establish a method for the determination of the dissolution of sarpogrelate hydrochloride tablets. METHODS: The dissolution of sarpogrelate hydrochloride tablets was determined by UV spectrophotometry with 0.1mol?L-1 hydrochloric acid as solvent at a rotation speed of 100r?min-1. The sampling time was 30min and the detection wavelength was set at 273nm. RESULTS: The linear range of sarpogrelate hydrochloride together was 3～30?g?mL-1(r=0.999 7) and the average recovery was 100%～100.35%, RSD=0.73%. The dissolutions of three batches of samples were all above 90%. CONCLUSION: The method is simple, accurate and reliable, and it can be used for the determination of the dissolution of sarpogrelate hydrochloride tablets.
ABSTRACT
OBJECTIVE: To establish a method for the determination of the dissolution of sitafloxacin tablets.METHODS: The dissolution sitafloxacin tablets was determined by peddle method in accordance with Chinese Pharmacopeia(2005 edition) using 0.1 mol?L-1 HCl as the medium with the rotation speed set at 75 r?min-1.Sampling was carried at 30 min with its absorbability determined by UV spectrophotometry under a wavelength of 295 nm and its dissolution rate calculated.RESULTS: The linear range of sitafloxacin was 2～20 ?g?mL-1(r=0.999 6) with an average recovery rate of 99.95%(RSD=0.40%).The dissolution rates of 3 batches of samples were all above 90%.CONCLUSION: The method is simple,accurate with reliable results,and it is applicable for the determination the dissolution rate of sitafloxacin tablets.